<p><b>BACKGROUND: </b>Biomarker variability, which includes within-individual variability (CV), between-individual variability (CV) and methodological variability (CV) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CV and CV may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n=58) and duplicate blood measurements (n=761-929 depending on the biomarker).</p><p><b>METHODS: </b>We estimated the index of individuality (II) ((CV+CV)/CV) for 41 analytes and evaluated differences in the II across sexes and age groups.</p><p><b>RESULTS: </b>Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18-44y age group as compared to the 45+y age group.</p><p><b>CONCLUSIONS: </b>The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.</p>